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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
18/09/2015 |
Data da última atualização: |
01/03/2016 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SILVA, C. G.; CUMPA, H. C. B.; FONSECA NETO, A. M. da; MARTINS, C. F.; BÁO, S. N. |
Afiliação: |
HEIDI CHRISTINA BESSLER CUMPA, CPAC; ALVARO MORAES DA FONSECA NETO, CPAC; CARLOS FREDERICO MARTINS, CPAC; UNB. |
Título: |
Effect of trichostatin a in cloned cattle embryo production by nuclear transfer with mesenchymal stem cells. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Animal Reproduction, v. 12, n. 3, p. 812, Jul./Sept. 2015. |
Idioma: |
Inglês |
Conteúdo: |
Acetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and further for 16 or 21 h in medium cultivation. Subsequently, the embryos continued in culture in synthetic oviductal fluid (SOF) medium without TSA and the parthenogenetic control was performed in every manipulation. Five NT procedures were performed for each treatment (20 h, 25 h and without TSA). Fusion rates, cleavage and blastocyst production were compared by Tukey test (p <0.05). The cleavage rate of parthenogenetic embryos (93.45 ± 7.97) was higher than the cleavage rate of embryos without treatment (82.45 ± 5.59) but was statistically similar to embryos treated with 20 and 25 h (87.25 ± 8.41 and 85.54 ± 3.88, respectively). Still, there was no difference in cleavage rate between treated and untreated embryos. The blastocyst production rate on the seventh day of culture was superior to the parthenogenetic control (59.24 ± 11.75) compared to treatments for 20 h (36.22 ± 16.80), 25 h (33.66 ± 12.84) and without the use of TSA (32.70 ± 9.11), which did not differ. It can be concluded that the use of TSA had no significant effect in improving the rates of cleavage and development of bovine embryos by nuclear transfer with MSCs from adipose tissue. However, additional studies to evaluate the quality and embryos of the methylation pattern should be conducted to better understand the effects of TSA in embryos cloned with this cell type. MenosAcetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and... Mostrar Tudo |
Palavras-Chave: |
Cloning; Epigenetic. |
Thesagro: |
Clonagem. |
Thesaurus Nal: |
histone deacetylase. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129960/1/34712.pdf
|
Marc: |
LEADER 03483nam a2200205 a 4500 001 2024379 005 2016-03-01 008 2015 bl uuuu u00u1 u #d 100 1 $aSILVA, C. G. 245 $aEffect of trichostatin a in cloned cattle embryo production by nuclear transfer with mesenchymal stem cells. 260 $aAnimal Reproduction, v. 12, n. 3, p. 812, Jul./Sept. 2015.$c2015 520 $aAcetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and further for 16 or 21 h in medium cultivation. Subsequently, the embryos continued in culture in synthetic oviductal fluid (SOF) medium without TSA and the parthenogenetic control was performed in every manipulation. Five NT procedures were performed for each treatment (20 h, 25 h and without TSA). Fusion rates, cleavage and blastocyst production were compared by Tukey test (p <0.05). The cleavage rate of parthenogenetic embryos (93.45 ± 7.97) was higher than the cleavage rate of embryos without treatment (82.45 ± 5.59) but was statistically similar to embryos treated with 20 and 25 h (87.25 ± 8.41 and 85.54 ± 3.88, respectively). Still, there was no difference in cleavage rate between treated and untreated embryos. The blastocyst production rate on the seventh day of culture was superior to the parthenogenetic control (59.24 ± 11.75) compared to treatments for 20 h (36.22 ± 16.80), 25 h (33.66 ± 12.84) and without the use of TSA (32.70 ± 9.11), which did not differ. It can be concluded that the use of TSA had no significant effect in improving the rates of cleavage and development of bovine embryos by nuclear transfer with MSCs from adipose tissue. However, additional studies to evaluate the quality and embryos of the methylation pattern should be conducted to better understand the effects of TSA in embryos cloned with this cell type. 650 $ahistone deacetylase 650 $aClonagem 653 $aCloning 653 $aEpigenetic 700 1 $aCUMPA, H. C. B. 700 1 $aFONSECA NETO, A. M. da 700 1 $aMARTINS, C. F. 700 1 $aBÁO, S. N.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Registros recuperados : 19 | |
3. | | MARTINS, C. F.; FERREIRA, I. C.; FONSECA NETO, A. M. da; GODOY, S. D.; TOLEDO, M. R. de; MIZIARA, F.; REIS, N. S. Quinta prova brasileira de produção de leite a pasto do zebu leiteiro no Centro de Tecnologia para Raças Zebuínas Leiteiras da Embrapa Cerrados: caderno de resultados para novilhas da raça Gir Leiteiro. Planaltina, DF: Embrapa Cerrados, 2021. 28 p. (Embrapa Cerrados. Documentos, 380).Biblioteca(s): Embrapa Cerrados. |
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4. | | MARTINS, C. F.; FERREIRA, I. C.; FONSECA NETO, A. M. da; TOLEDO, M. R.; MIZIARA, F.; BALBINO, L. C.; GODOY, S. D. Quarta prova brasileira de produção de leite a pasto do zebu leiteiro no Centro de Tecnologias para Raças Zebuínas Leiteiras da Embrapa Cerrados: caderno de resultados para novilhas da raça Gir Leiteiro Planaltina, DF: Embrapa Cerrados, 2020. 39 p. (Embrapa Cerrados. Documentos, 358).Biblioteca(s): Embrapa Cerrados. |
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5. | | BISCAÍNO, L. L.; ROCHA, M. G.; POTTER, L.; ELOY, L. R.; FONSECA NETO, A. M. da; ALVES, M. B.; GRAMINHO, L. A.; SICHONANY, M. J. O. Desempenho de bezerras de corte em pastagem de azevém recebendo farelo de arroz com ou sem monensina. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 70, n. 3, 2018. p. 881-887Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Cerrados. |
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7. | | FIGUEIREDO, P. O.; AZAMBUJA, G. G.; BARROS, L. C.; CUMPA, H. C. B.; FONSECA NETO, A. M. da; PIVATO, I.; MARTINS, C. F. Ultrasound evaluation of ovarian dynamics in Indubrasil cows submitted to two nutritional managements. Animal Reproduction, v. 12, n. 3, p. 571, Jul./Sept. 2015.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Cerrados. |
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8. | | ALVES, M. B.; ROCHA, M. G.; POTTER, L.; STIVANIN, S. C. B.; HAMPEL, V. S.; FONSECA NETO, A. M. da; SICHONANY, M. J. O.; MOTERLE, P. H. Uso de suplementos para acasalamento de bezerras Angus aos 14 meses de idade. Use of supplements for mating Angus heifers at 14 months. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 68, n. 3, p. 755-760, mai./jun. 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Cerrados. |
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9. | | FERREIRA, I. C.; SILVA, P. M. R. S.; MALAQUIAS, J. V.; FONSECA NETO, A. M. da; OLIVEIRA, S. A. S.; PINHO, G. A. S.; MARTINS, C. F. Habituação de novilhas Zebuínas no pré-parto para entrar na sala de ordenha: avaliação qualitativa. Planaltina, DF : Embrapa Cerrados, 2020. 22 p. (Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 365).Tipo: Boletim de Pesquisa e Desenvolvimento |
Biblioteca(s): Embrapa Cerrados. |
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10. | | SILVA, C. G. da; BÁO, S. N.; CUMPA, H. C. B.; CARDOSO, T. C.; FONSECA, L. O. da; FONSECA NETO, A. M. da; GODOY, S. D.; MARTINS, G. H.; CUNHA, E. R. da; MARTINS, C. F. Brasília da Cerrados: primeiro bovino clonado utilizando células-tronco mesenquimais do tecido adiposo. Planaltina, DF: Embrapa Cerrados, 2016. 34 p. (Embrapa Cerrados. Documentos, 330)Biblioteca(s): Embrapa Cerrados. |
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11. | | FERREIRA, I. C.; FONSECA NETO, A. M. da; MULLER, A. G.; MARTINS, C. F.; SILVA, F. A. M. da; BRAGA, G. J.; MALAQUIAS, J. V.; PULROLNIK, K.; VILELA, L.; BALBINO, L. C.; GUIMARAES JUNIOR, R. Produção de Leite em Sistema de Integração Lavoura-Pecuária- Floresta (ILPF) no Bioma Cerrado. Planaltina, DF: Embrapa Cerrados, 2021. 26 p. (Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 375).Tipo: Boletim de Pesquisa e Desenvolvimento |
Biblioteca(s): Embrapa Cerrados. |
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12. | | MARTINS, C. F.; FERREIRA, I. C.; TOLEDO, M. R. de; MACHADO, C. H. C.; FONSECA NETO, A. M. da; BALBINO, L. C.; GODOY, S. D.; PACHECO, G. R.; CUMPA, H. C. B. Primeira prova brasileira de produção de leite a pasto do Zebu Leiteiro no Centro de Tecnologias para Raças Zebuínas Leiteiras (CTZL) da Embrapa Cerrados: caderno de resultados para novilhas da raça Gir Leiteiro Planaltina, DF: Embrapa Cerrados, 2016. 41 p. (Embrapa Cerrados. Documentos, 336).Biblioteca(s): Embrapa Cerrados; Embrapa Unidades Centrais. |
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13. | | MARTINS, C. F.; FONSECA NETO, A. M. da; BESSLER, H. C.; DODE, M. A. N.; LEME, L. O.; FRANCO, M. M.; McMANUS, C. M.; MALAQUIAS, J. V.; FERREIRA, I. C. Natural shade from integrated crop livestock forestry mitigates environmental heat and increases the quantity and quality of oocytes and embryos produced in vitro by Gyr dairy cows. Livestock Science, 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Cerrados. |
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14. | | MARTINS, C. F.; FONSECA NETO, A. M. da; BESSLER, H. C.; DODE, M. A. N.; LEME, L. O.; FRANCO, M. M.; McMANUS, C. M.; MALAQUIAS, J. V.; FERREIRA, I. C. Natural shade from integrated crop livestock forestry mitigates environmental heat and increases the quantity and quality of oocytes and embryos produced in vitro by Gyr dairy cows. Livestock Science, v. 244, 104341, 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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15. | | MARTINS, C. F.; FERREIRA, I. C.; FONSECA NETO, A. M. da; TOLEDO, M. R. de; OJEDA, D. B.; GODOY, S. D.; PACHECO, G. R.; CUMPA, H. C. B.; BORGES, J. R. J.; BALBINO, L. C. Segunda prova brasileira de produção de leite a pasto do zebu leiteiro no Centro de Tecnologias para Raças Zebuínas Leiteiras da Embrapa Cerrados: caderno de resultados para vacas e novilhas da raça Gir Leiteiro e para novilhas Sindi. Planaltina, DF: Embrapa Cerrados, 2018. 55 p. (Embrapa Cerrados. Documentos, 345).Biblioteca(s): Embrapa Cerrados. |
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16. | | FONSECA NETO, A. M. da; MARTINS, C. F.; MIZIARA, F.; PACHECO, G. R.; CUMPA, H. C. B.; FERREIRA, I. C.; BORGES, J. R. J.; BALBINO, L. C.; TOLETO, M. R. de; SILVA, P. M. R. S. da; GODOY, S. D. Terceira prova brasileira de produção de leite a pasto do Zebu Leiteiro no Centro de Tecnologias para Raças Zebuínas Leiteiras (CTZL) da Embrapa Cerrados: caderno de resultados para novilhas da raça Gir Leiteiro Planaltina, DF: Embrapa Cerrados, 2019. 41 p. (Embrapa Cerrados. Documentos, 351).Biblioteca(s): Embrapa Cerrados. |
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17. | | SILVA, C. G. da; MARTINS, C. F.; CUMPA, H. C. B.; FONSECA NETO, A. M. da; CARDOSO, T. C.; FRANCO, M. M.; MENDONÇA, A. dos S.; LEME, L. de O.; BORGES, J. R. J.; MALAQUIAS, J. V.; BAO, S. N. Use of trichostatin A alters the expression of HDAC3 and KAT2 and improves in vitro development of bovine embryos cloned using less methylated mesenchymal stem cells. Reproduction in Domestic Animals, v. 54, n. 2, p. 289-299, February 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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18. | | CARDOSO, C. C.; PERIPOLLI, V.; AMADOR, S. A.; BRANDÃO, E. G.; ESTEVES, G. I. F.; SOUSA, C. M. Z.; FRANÇA, M. F. M. S.; GONÇALVES, F. G.; BARBOSA, F. A.; MONTALVÃO, T. C.; MARTINS, C. F.; FONSECA NETO, A. M. da; MCMANUS, C. Physiological and thermographic response to heat stress in zebu cattle. Livestock Science, v. 182, p. 83-92, DEC. 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Cerrados. |
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19. | | REIS, N. S.; FERREIRA, I. C.; MAZOCOO, L. A.; SOUZA, A. C. B.; PINHO, G. A. S.; FONSECA NETO, A. M. da; MALAQUIAS, J. V.; SILVA, F. A. M. da; MULLER, A. G.; MARTINS, C. F.; BALBINO, L. C.; MCMANUS, C. M. Shade Modifies Behavioral and Physiological Responses of Low to Medium Production Dairy Cows at Pasture in an Integrated Crop-Livestock-Forest System. Animals, v. 11, n. 2411, 2021. 12 p.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Cerrados. |
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Registros recuperados : 19 | |
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